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Wednesday, 22 February 2012 19:14
RMV's dna double-checked Print E-mail
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After the failed Bergman analysis shall Forensic Medicine in the future to compare all DNA test results with DNA from employees and visitors.
 
 
When Forensic Medicine, RMV, in Linkoping next fall started using a new data support the plant all DNA analysis will automatically be compared with DNA from staff and other temporary visitors to the laboratory.

The aim is to avoid DNA from people who spend time in the lab mixed up with or "contaminating" DNA analysis of the material, which may lead to the RMV disclosing false information to its various clients.

Forensic Medicine is recently completed its investigation into how a DNA from a biomedical technician could rub off on DNA samples of stamps from director Ingemar Bergman.

The RMV did was to analyze sensitive mitikondrie DNA, small pieces of cell debris, which were on the stamps.

Frimärksproverna however, became contaminated with the technician's DNA on three separate occasions. As a result of this was false dna response to the director's niece Veronica Ralston, who had given RMV commissioned to examine the relationship with his uncle.

The new routine to check for new DNA-response versus employees and visitors will also include responses to nuclear DNA, says Johan Ahlner, Head of Forensic Toxicology and forensic genetics at the RMV.

We now have the ability to do this and it would be foolish not to implement the function. This raises of course the security, he says.

Until autumn 2012, it will compare the DNA-response produced by the staff dna, only in cases that seem suspicious for any reason.

The staff is already tops and their DNA profiles are complete. Exactly what other groups that may be present in the labs for various reasons and for that reason may have to provide DNA samples are Office lawyer to go through, according to investigators RMVs Andreas Tillmar.  But it can involve, for example technicians and craftsmen.

RMV is that part of its quality assessment testing to allow various laboratory techniques to make new mitochondrial DNA analysis on stamps.  Even in these cases it has been shown that several employees contaminated DNA samples.

In the majority of cases, the problems arise from the extraction of DNA in the lab, said Andreas Tillmar, who was also responsible for parts of the Bergman-analysis together with another DNA expert.

According to Board inquiry is the risk of contamination during the analysis "very high",

The routines of the method will now be reviewed. The Agency will not perform any new similar analysis before you made sure that it is safe, said John Ahlner.  He stresses that the RMV does not have disclosed the incorrect answers in some cases other than in the Bergman case.

Agency Director General are Zelmin Ekenhem has previously said that the analytical method has not been fully developed and therefore never would have come inside the doors of the RMV.

But John Ahlner says it will continue to develop it, because in the future may need to make use of mitochondrial DNA analysis, such as the identification of dead, unknown persons.

He is however doubtful that it will analyze the DNA of just sticking stamps to come.

Administration has also, before Bergman assays, previously made mitikondrie DNA analysis to investigate relationships.
We have used it as an additional method of analysis in some kinship studies, but then we also had access to nuclear DNA and then the risk of contamination is almost nonexistent, says Andreas Tillmar.
 
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